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A FILTRATION PROCEDURE FOR DETERMINING SERUM CHOLESTEROL WITH DIGITONIN
Author(s) -
Brown WD
Publication year - 1961
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1961.23
Subject(s) - digitonin , saponification , chromatography , chemistry , reagent , filtration (mathematics) , centrifugation , cholesterol , decantation , biochemistry , organic chemistry , mathematics , enzyme , statistics
SUMMARY A precise and accurate method was developed for the determination of cholesterol with Liebermann‐Burchard reagent after its isolation as the digitonide. The cholesteryl digitonide was isolated by filtration instead of by the usual procedure of repeated centrifugation and decantation of wash fluids. The method is as precise as methods in which colorimetric reactions are applied to the non‐saponifiable extract and in which accuracy is obtained with the preparation of internal standards. Parameters are given for the maximum amounts of cholesteryl esters which may be saponified at 37° C. over a 30‐minute period. The difficulties of saponifying cholesteryl stearate due to its insolubility are discussed. It is shown that a protracted period of saponification at 65° C. need not affect the accuracy of determination of cholesterol with digitonin.