Premium
THE ANALYSIS OF ELECTROPHORESIS PATTERNS: A COMPARISON OF TWO METHODS WITH THE AID OF RAYLEIGH INTERFERENCE OPTICS
Author(s) -
Curtain CC
Publication year - 1961
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1961.20
Subject(s) - interference (communication) , electrophoresis , resolution (logic) , optics , maxima and minima , gaussian , point (geometry) , chromatography , physics , mathematics , chemistry , computer science , artificial intelligence , geometry , mathematical analysis , channel (broadcasting) , quantum mechanics , computer network
SUMMARY Two methods of serum electrophoresis pattern resolution have been investigated: (a) the method of Kabat and Tiselius (1939) in which the components are delimited by dropping perpendiculars from minima in the gradient curve; (b) the method of Svedberg and Pedersen (1940) in which the pattern is resolved into Gaussian curves, the sum of whose ordinates at any given point is equal to the ordinate of the gradient curve at that point. It was found that the latter method was the less influenced by the degree of separation of the components but it is inconvenient to use with interferometric optical systems because it is necessary to plot the entire gradient curve. It was found possible to use nearly all the cell height of the Perkin‐Elmer model 38A electrophoresis instrument if the plateholder were modified to ensure reproducible location of the plate at each exposure. This made it possible to carry out electrophoresis for a time which gave sufficient separation of the components to enable the method of Kabat and Tiselius to be used.