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THE EFFECT OF CHLORAMPHENICOL AND OXYTETRACYCLINE ON THE FORMATION OF INTERMEDIATES IN TRYPTOPHAN BIOSYNTHESIS
Author(s) -
Gibson F,
McDougall Barbara
Publication year - 1961
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1961.17
Subject(s) - chloramphenicol , oxytetracycline , tryptophan , anthranilic acid , antibiotics , biosynthesis , escherichia coli , indole test , chemistry , mode of action , biochemistry , microbiology and biotechnology , biology , amino acid , enzyme , gene
SUMMARY Previous experiments on the effects of chloramphenicol and the tetracyclines on the biosynthesis of tryptophan in Escherichia coli have been extended using a number of tryptophan auxotrophs. The formation of indole, anthranilic acid and 5‐dehydroshikimic acid is inhibited by chloramphenicol and oxytetracycline. There is some variation among different strains with respect to the sensitivity of the aromatic pathway to the antibiotics. It is suggested that the antibiotics possibly interfere in some reaction leading to the formation of 5‐dehydroshikimic acid. The present results provide further evidence that the mode of action of chloramphenicol and of the tetracyclines are related in some way.