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A HEAT STABLE PROTECTIVE STAPHYLOCOCCAL ANTIGEN
Author(s) -
Fisher Stephen
Publication year - 1960
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1960.53
Subject(s) - microbiology and biotechnology , virulence , antigen , mucin , strain (injury) , intraperitoneal injection , agar , chemistry , biology , bacteria , immunology , pharmacology , biochemistry , genetics , anatomy , gene
SUMMARY A strain of staphylococci, chosen for the very marked enhancement by mucin of its intraperitoneal virulence to mice, was cultured in sloppy agar. The supernatant fluids obtained were of low toxicity and were highly potent as active immunizing agents in mice against lethal intraperitoneal challenge by the homologous strain in mucin suspension. The activity of the protective antigen was not affected by boiling for 10 minutes and was retained in the bag during dialysis. The protection could be transferred passively by means of the sera of immunized animals.