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THE AMOUNT OF PLASMA TRAPPED BY RED CELLS IN CENTRIFUGED AND SEDIMENTED BLOOD
Author(s) -
Clark Peggy,
Walsh RJ
Publication year - 1960
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1960.49
Subject(s) - centrifugation , sedimentation , glass tube , chemistry , chromatography , tube (container) , plasma , centrifuge , red blood cell , red cell , analytical chemistry (journal) , materials science , composite material , biology , biochemistry , physics , medicine , paleontology , quantum mechanics , sediment , nuclear physics
SUMMARY An accurate method for dividing red blood cell columns has been devised. Blood is placed in P.V.C. tubing which is sealed at one end with a glass bead. This tubing is inserted into a piece of glass tube before centrifugation, and later the contents can be frozen before the tubing is divided. The method was used to measure the amount of plasma trapped with the red blood cells. From these measurements the ratio of the true to the observed haematocrit value was found to be 0·94 at centrifugal forces greater than 1,430 g. The ratio decreased with decreasing relative centrifugal force, but variations in the haematocrit value did not alter the correction factor. The amount of plasma trapped in centrifuged and sedimented normal human blood decreased from top to bottom of the red cell columns, but the amount in different sections of the columns became more uniform as the time of centrifugation or of sedimentation increased. A blood sample with a high sedimentation rate showed a different pattern on sedimentation.