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BLOOD pH DETERMINATION
Author(s) -
Holden Henry Francis
Publication year - 1960
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1960.45
Subject(s) - pipette , electrode , saturated calomel electrode , capillary action , tap water , analytical chemistry (journal) , materials science , volt , mercury (programming language) , chemistry , chromatography , reference electrode , composite material , voltage , electrical engineering , environmental science , electrolyte , environmental engineering , engineering , computer science , programming language
SUMMARY The paper describes a simple and inexpensive apparatus for the measurement of pH at 38° using only 0·15 to 0·2 ml. of blood. The electrode bulb nearly fills the bore of the electrode vessel. There is a wick junction but no tap between the 3·5 M KCl calomel half‐cell and the electrode vessel. This last is filled and emptied with bent capillary pipettes. The bath contains kerosene heated with a bare coil which receives 2 amp. at 12 volts. It is controlled with a small mercury‐in‐glass regulator and a relay.
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