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DETERMINATION OF PROTEIN FRACTIONS IN SMALL SAMPLES OF PLASMA AND OF LYMPH IN THE RABBIT
Author(s) -
Courtice FC
Publication year - 1960
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1960.42
Subject(s) - albumin , globulin , chromatography , chemistry , lymph , kjeldahl method , blood proteins , fibrinogen , bromophenol blue , biochemistry , biology , endocrinology , medicine , pathology , organic chemistry , nitrogen
SUMMARY In an investigation of the transfer of proteins and lipids across the capillary membrane in the rabbit, only small volumes of lymph were often available. This necessitated the use of a method for determining the several protein fractions in lymph which involved the use of such small volumes. Total protein was determined by kjeldahl digestion and nesslerization, and the protein fractions by paper electrophoresis. The various steps in the procedure of electrophoresis were standardized and the dye‐binding capacities of the globulin fractions were determined in relation to albumin. All the globulin fractions were shown to bind less dye, bromophenol blue, than albumin and the areas of the dye curves were multiplied by the following factors in relation to albumin as unity: γ ‐globulin and fibrinogen, 1·76; β ‐globulin 1·40, α ‐globulin 1·50 and total globulin 1·56. The accuracy of the method for the determination of the levels of these fractions in the plasma and lymph of normal and hypercholesterolaemic rabbits is discussed.