Premium
SOME OBSERVATIONS ON THE PRODUCTION OF BILE BY THE ISOLATED PERFUSED LIVER OF THE RAT
Author(s) -
Morris Bede
Publication year - 1960
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1960.11
Subject(s) - perfusion , medicine , chemistry , endocrinology , blood flow , oxygen , secretion , biology , organic chemistry
SUMMARY A method for perfusing the isolated liver of the rat in a gas‐tight system is described. Under the conditions of the experiments, the livers produced bile and metabolized fat for several hours. Some observations on the effect of temperature, perfusion pressure and the concentration of fat in the perfusate on the production of bile are reported. It was found that the production of bile was maximal at 38°C. At temperatures of 32 and 42°C., bile flow was significantly reduced. The volume of bile produced was related to the perfusion pressure and increased as the pressure was raised from 5 to 25 cm. of blood. The concentration of fat in the perfusate had no effect on bile production. There was a significant interaction effect between temperature and perfusion pressure which was thought to be explained by the oxygen requirements of the liver cells in relation to blood flow and bile secretion. The results suggested that bile secretion reached a maximum when all the hepatic sinuses were perfused, provided the oxygen supply to the liver cells was adequate.