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THE DIFFERENTIAL STAINING OF HUMAN AND DOG SPERMATOZOA
Author(s) -
Wales RG
Publication year - 1959
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1959.44
Subject(s) - stain , eosin , staining , semen , trypan blue , h&e stain , differential staining , significant difference , sperm , andrology , biology , anatomy , medicine , in vitro , biochemistry , genetics
SUMMARY Human spermatozoa can be stained successfully with trypan blue, eosin and erythrosin and although each stain gives a difference in the estimate of “live” cells in a sample, this difference is consistent for each ejaculate, before and after heat treatment. It is suggested that because of the relative simplicity of the procedure, eosin‐nigrosin should be used routinely to stain human spermatozoa. Eosin‐nigrosin should be used in preference to congo red‐nigrosin as a vital stain for dog spermatozoa as it gives better differentiation, a variance estimate which is more consistent with that expected from theory and less difference between operators. The percentage of stained spermatozoa in a semen‐eosin mixture has been found to increase with time of exposure to the stain. This effect was seen with both dog and human spermatozoa.