Open AccessCaveolin-1 upregulation in senescent neurons alters amyloid precursor protein processing
Author(s) -
Min-Ji Kang,
Yoon Hee Chung,
ChangIl Hwang,
Michiyo Murata,
Toyoshi Fujimoto,
In-Hee Mook-Jung,
Choong Ik,
WoongYang Park
Publication year - 2006
Publication title -
experimental and molecular medicine
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 2.703
H-Index - 82
eISSN - 2092-6413
pISSN - 1226-3613
DOI - 10.1038/emm.2006.16
Subject(s) - downregulation and upregulation , caveolin 1 , caveolae , microbiology and biotechnology , amyloid precursor protein , protein kinase c , hippocampus , cerebral cortex , biology , lipid raft , kinase , neuroscience , chemistry , signal transduction , alzheimer's disease , biochemistry , medicine , gene , disease
Lipid rafts provide a platform for regulating cellular functions and participate in the pathogenesis of several diseases. However, the role of caveolin-1 in this process has not been elucidated definitely in neuron. Thus, this study was performed to examine whether caveolin-1 can regulate amyloid precursor protein (APP) processing in neuronal cells and to identify the molecular mechanisms involved in this regulation. Caveolin-1 is up-regulated in all parts of old rat brain, namely hippocampus, cerebral cortex and in elderly human cerebral cortex. Moreover, detergent-insoluble glycolipid (DIG) fractions indicated that caveolin-1 was co-localized with APP in caveolae-like structures. In DIG fractions, beta APP secretion was up-regulated by caveolin-1 over- expression, which was modulated via protein kinase C (PKC) in neuroblastoma cells. From these results we conclude that caveolin-1 is selectively expressed in senescent neurons and that it induces the processing of APP by beta-secretase via PKC downregulation.
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