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Adenosine‐A 3 receptors in neutrophil microdomains promote the formation of bacteria‐tethering cytonemes
Author(s) -
Corriden Ross,
Self Tim,
AkongMoore Kathryn,
Nizet Victor,
Kellam Barrie,
Briddon Stephen J,
Hill Stephen J
Publication year - 2013
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/embor.2013.89
Subject(s) - chemotaxis , microbiology and biotechnology , phagocytosis , receptor , agonist , tethering , chemistry , adenosine receptor , regulator , adenosine , biology , biophysics , biochemistry , gene
The A 3 ‐adenosine receptor (A 3 AR) has recently emerged as a key regulator of neutrophil behaviour. Using a fluorescent A 3 AR ligand, we show that A 3 ARs aggregate in highly polarized immunomodulatory microdomains on human neutrophil membranes. In addition to regulating chemotaxis, A 3 ARs promote the formation of filipodia‐like projections (cytonemes) that can extend up to 100 μm to tether and ‘reel in’ pathogens. Exposure to bacteria or an A 3 AR agonist stimulates the formation of these projections and bacterial phagocytosis, whereas an A 3 AR‐selective antagonist inhibits cytoneme formation. Our results shed new light on the behaviour of neutrophils and identify the A 3 AR as a potential target for modulating their function.