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Antisense regulation by transposon‐derived RNAs in the hyperthermophilic archaeon Sulfolobus solfataricus
Author(s) -
Märtens Birgit,
Manoharadas Salim,
Hasenöhrl David,
Manica Andrea,
Bläsi Udo
Publication year - 2013
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/embor.2013.47
Subject(s) - sulfolobus solfataricus , biology , rna , transposable element , antisense rna , untranslated region , messenger rna , sulfolobus , three prime untranslated region , gene , microbiology and biotechnology , genetics , archaea , mutant
We report the first example of antisense RNA regulation in a hyperthermophilic archaeon. In Sulfolobus solfataricus , the transposon‐derived paralogous RNAs, RNA‐257 1–4 , show extended complementarity to the 3′ UTR of the 1183 mRNA, encoding a putative phosphate transporter. Phosphate limitation results in decreased RNA‐257 1 and increased 1183 mRNA levels. Correspondingly, the 1183 mRNA is faster degraded in vitro upon duplex formation with RNA‐257 1 . Insertion of the 1183 3′ UTR downstream of the lacS gene results in strongly reduced lacS mRNA levels in transformed cells, indicating that antisense regulation can function in trans .