Antisense regulation by transposon‐derived RNAs in the hyperthermophilic archaeon  Sulfolobus solfataricus | Zendy

Märtens Birgit | Zendy; Manoharadas Salim | Zendy; Hasenöhrl David | Zendy; Manica Andrea | Zendy; Bläsi Udo | Zendy

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Antisense regulation by transposon‐derived RNAs in the hyperthermophilic archaeon Sulfolobus solfataricus

Author(s) -

Märtens Birgit,

Manoharadas Salim,

Hasenöhrl David,

Manica Andrea,

Bläsi Udo

Publication year - 2013

Publication title -

embo reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 4.584

H-Index - 184

eISSN - 1469-3178

pISSN - 1469-221X

DOI - 10.1038/embor.2013.47

Subject(s) - sulfolobus solfataricus , biology , rna , transposable element , antisense rna , untranslated region , messenger rna , sulfolobus , three prime untranslated region , gene , microbiology and biotechnology , genetics , archaea , mutant

We report the first example of antisense RNA regulation in a hyperthermophilic archaeon. In Sulfolobus solfataricus , the transposon‐derived paralogous RNAs, RNA‐257 1–4 , show extended complementarity to the 3′ UTR of the 1183 mRNA, encoding a putative phosphate transporter. Phosphate limitation results in decreased RNA‐257 1 and increased 1183 mRNA levels. Correspondingly, the 1183 mRNA is faster degraded in vitro upon duplex formation with RNA‐257 1 . Insertion of the 1183 3′ UTR downstream of the lacS gene results in strongly reduced lacS mRNA levels in transformed cells, indicating that antisense regulation can function in trans .

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