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Aurora A kinase and its substrate TACC3 are required for central spindle assembly
Author(s) -
Lioutas Antonios,
Vernos Isabelle
Publication year - 2013
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/embor.2013.109
Subject(s) - anaphase , microbiology and biotechnology , spindle checkpoint , aurora a kinase , spindle apparatus , mitosis , aurora b kinase , spindle pole body , anaphase promoting complex , chromosome segregation , plk1 , multipolar spindles , biology , cell division , chemistry , cell cycle , genetics , cell , chromosome , gene
Cell division entails a marked reorganization of the microtubule network to form the spindle, a molecular machine that ensures accurate chromosome segregation to the daughter cells. Spindle organization is highly dynamic throughout mitosis and requires the activity of several kinases and complex regulatory mechanisms. Aurora A (AurA) kinase is essential for the assembly of the metaphase bipolar spindle and, thus, it has been difficult to address its function during the last phases of mitosis. Here, we examine the consequences of inhibiting AurA in cells undergoing anaphase, and show that AurA kinase activity is necessary for the assembly of a robust central spindle during anaphase. We also identify TACC3 as an AurA substrate essential in central spindle formation.