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Tankyrase 1 regulates centrosome function by controlling CPAP stability
Author(s) -
Kim Mi Kyung,
Dudog Charles,
Smith Susan
Publication year - 2012
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/embor.2012.86
Subject(s) - centrosome , microbiology and biotechnology , function (biology) , biology , genetics , gene , cell cycle
CPAP—a gene mutated in primary microcephaly—is required for procentriole formation. Here we show that CPAP degradation and function is controlled by the poly(ADP‐ribose) polymerase tankyrase 1. CPAP is PARsylated by tankyrase 1 in vitro and in vivo . Overexpression of tankyrase 1 leads to CPAP proteasomal degradation, preventing centriole duplication, whereas depletion of tankyrase 1 stabilizes CPAP in G1, generating elongated procentrioles and multipolarity. Tankyrase 1 localizes to centrosomes exclusively in G1, coinciding with CPAP degradation. Hence, tankyrase 1‐mediated PARsylation regulates CPAP levels during the cell cycle to limit centriole elongation and ensure normal centrosome function.