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Neurl4, a novel daughter centriole protein, prevents formation of ectopic microtubule organizing centres
Author(s) -
Li Ji,
Kim Sehyun,
Kobayashi Tetsuo,
Liang FengXia,
Korzeniewski Nina,
Duensing Stefan,
Dynlacht Brian D
Publication year - 2012
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/embor.2012.40
Subject(s) - microbiology and biotechnology , centrosome , mitosis , microtubule organizing center , biology , centriole , ectopic expression , microtubule , genetics , cell cycle , apoptosis , cell culture
Here we identify Neuralized homologue 4 (Neurl4) as a protein that interacts with CP110, a centrosomal protein that regulates centrosome duplication. Neurl4 uses a Neuralized homology repeat to preferentially localize to procentrioles and daughter centrioles. Neurl4 depletion results in ectopic microtubular organizing centres (MTOCs), leading to accumulation of CP110 and recruitment of a cohort of centrosomal proteins. We show that these ectopic MTOCs persist through mitosis and assemble aberrant mitotic spindles. Interestingly, Neurl4 promotes ubiquitylation of CP110, thereby destabilizing this protein. Our results indicate that Neurl4 counteracts accumulation of CP110, thereby maintaining normal centriolar homeostasis and preventing formation of ectopic MTOCs.