Premium
Open and closed domains in the mouse genome are configured as 10‐nm chromatin fibres
Author(s) -
Fussner Eden,
Strauss Mike,
Djuric Ugljesa,
Li Ren,
Ahmed Kashif,
Hart Michael,
Ellis James,
BazettJones David P
Publication year - 2012
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/embor.2012.139
Subject(s) - chromatin , genome , microbiology and biotechnology , biology , computational biology , genetics , dna , gene
The mammalian genome is compacted to fit within the confines of the cell nucleus. DNA is wrapped around nucleosomes, forming the classic ‘beads‐on‐a‐string’ 10‐nm chromatin fibre. Ten‐nanometre chromatin fibres are thought to condense into 30‐nm fibres. This structural reorganization is widely assumed to correspond to transitions between active and repressed chromatin, thereby representing a chief regulatory event. Here, by combining electron spectroscopic imaging with tomography, three‐dimensional images are generated, revealing that both open and closed chromatin domains in mouse somatic cells comprise 10‐nm fibres. These findings indicate that the 30‐nm chromatin model does not reflect the true regulatory structure in vivo .