Premium
Role of the import motor in insertion of transmembrane segments by the mitochondrial TIM23 complex
Author(s) -
PopovČeleketić Dušan,
Waegemann Karin,
Mapa Koyeli,
Neupert Walter,
Mokranjac Dejana
Publication year - 2011
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/embor.2011.72
Subject(s) - microbiology and biotechnology , translocase of the inner membrane , inner mitochondrial membrane , motor protein , biology , chromosomal translocation , transmembrane protein , mitochondrion , mitochondrial carrier , biophysics , chemistry , mitochondrial membrane transport protein , biochemistry , bacterial outer membrane , receptor , microtubule , gene , escherichia coli
The TIM23 complex mediates translocation of proteins across, and their lateral insertion into, the mitochondrial inner membrane. Translocation of proteins requires both the membrane‐embedded core of the complex and its ATP‐dependent import motor. Insertion of some proteins, however, occurs in the absence of ATP, questioning the need for the import motor during lateral insertion. We show here that the import motor associates with laterally inserted proteins even when its ATPase activity is not required. Furthermore, our results suggest a role for the import motor in lateral insertion. Thus, the import motor is involved in ATP‐dependent translocation and ATP‐independent lateral insertion.