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The role of deimination in ATP5b mRNA transport in a transgenic mouse model of multiple sclerosis
Author(s) -
Ding Di,
EnriquezAlgeciras Mabel,
Dave Kunjan R,
PerezPinzon Miguel,
Bhattacharya Sanjoy K
Publication year - 2012
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/embor.2011.264
Subject(s) - citrullination , citrulline , messenger rna , biology , arginine , mitochondrion , microbiology and biotechnology , chemistry , biochemistry , gene , amino acid
Deimination refers to conversion of protein‐bound arginine into citrulline. An mRNA carrier, RNA binding export factor (REF), present on mitochondria undergoes loss of deimination with impaired ATP5b mRNA transport in ND4 mice (model of multiple sclerosis) compared with the controls. We present evidence of (1) reduced ATP5b mRNA binding strength of non‐deiminated REF compared with deiminated REF, (2) impaired ATP5b mRNA transport in ND4 mice and (3) reduced mitochondrial ATP synthase activity on inhibition of deimination in PC12 cells. Impaired deimination of REF and defect in mitochondrial mRNA transport are critical factors in mitochondrial dysfunction in ND4 mice.