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ChAM, a novel motif that mediates PALB2 intrinsic chromatin binding and facilitates DNA repair
Author(s) -
Bleuyard JeanYves,
Buisson Rémi,
Masson JeanYves,
Esashi Fumiko
Publication year - 2012
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/embor.2011.243
Subject(s) - chromatin , palb2 , rad51 , biology , homologous recombination , genetics , dna damage , dna repair , dna , microbiology and biotechnology , gene , mutation , germline mutation
The partner and localizer of breast cancer 2 susceptibility protein (PALB2) is crucial for the repair of DNA damage by homologous recombination. Here, we report that chromatin‐association motif (ChAM), an evolutionarily conserved motif in PALB2, is necessary and sufficient to mediate its chromatin association in both unperturbed and damaged cells. ChAM is distinct from the previously described PALB2 DNA‐binding regions. Deletion of ChAM decreases PALB2 and Rad51 accumulation at DNA damage sites and confers cellular hypersensitivity to the genotoxic drug mitomycin C. These results suggest that PALB2 chromatin association via ChAM facilitates PALB2 function in the cellular resistance to DNA damage.