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Acetylation regulates monopolar attachment at multiple levels during meiosis I in fission yeast
Author(s) -
Kagami Ayano,
Sakuno Takeshi,
Yamagishi Yuya,
Ishiguro Tadashi,
Tsukahara Tatsuya,
Shirahige Katsuhiko,
Tanaka Koichi,
Watanabe Yoshinori
Publication year - 2011
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/embor.2011.188
Subject(s) - cohesin , centromere , establishment of sister chromatid cohesion , kinetochore , meiosis , microbiology and biotechnology , biology , genetics , chromosome segregation , meiosis ii , chromosome , gene
In fission yeast, meiotic mono‐orientation of sister kinetochores is established by cohesion at the core centromere, which is established by a meiotic cohesin complex and the kinetochore protein Moa1. The cohesin subunit Psm3 is acetylated by Eso1 and deacetylated by Clr6. We show that in meiosis, Eso1 is required for establishing core centromere cohesion during S phase, whereas Moa1 is required for maintaining this cohesion after S phase. The clr6‐1 mutation suppresses the mono‐orientation defect of moa1Δ cells, although the Clr6 target for this suppression is not Psm3. Thus, several acetylations are crucial for establishing and maintaining core centromere cohesion.