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A genetic in vivo system to detect asymmetrically distributed RNA
Author(s) -
JayaNandanan N,
Gavis Elizabeth R,
Riechmann Veit,
Leptin Maria
Publication year - 2011
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/embor.2011.178
Subject(s) - rna , messenger rna , biology , subcellular localization , genome , gene , computational biology , microbiology and biotechnology , in vivo , gene expression , genetics
Many RNAs show polarized or otherwise non‐random subcellular distributions. To create a method for genome‐wide genetic screens for RNAs with asymmetric subcellular distributions, we have combined methods for gene tagging and live imaging of messenger RNA (mRNA). A pilot screen in a highly polarized, differentiated cell in the Drosophila larva, the branched terminal cell of the tracheal system, demonstrates the feasibility of the method for identifying new asymmetrically localized mRNAs in vivo .