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Lac operator repeats generate a traceable fragile site in mammalian cells
Author(s) -
Jacome Ariana,
FernandezCapetillo Oscar
Publication year - 2011
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/embor.2011.158
Subject(s) - chromosomal fragile site , anaphase , helicase , metaphase , biology , mitosis , genetics , interphase , chromatid , microbiology and biotechnology , cell cycle , chromosome , cell , gene , rna
One limitation for the study of chromosomal fragile sites is that they must be studied on metaphase spreads, after the breakage. We show here that bacterial lac operator (lacO) repeats are prone to spontaneous breakage, which when combined with a fluorescent lac repressor (lacR) has allowed us to track a fragile site through the cell cycle. By using this system, we show that Plk1‐interacting checkpoint helicase (PICH) is already present at fragile sites during interphase, suggesting roles for this helicase beyond mitosis. In addition, we report that the oncogene Myc promotes the formation of anaphase bridges and micronuclei containing fragile‐site sequences.