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Polo‐like kinase 1 phosphorylation of G2 and S‐phase‐expressed 1 protein is essential for p53 inactivation during G2 checkpoint recovery
Author(s) -
Liu X Shawn,
Li Hongchang,
Song Bing,
Liu Xiaoqi
Publication year - 2010
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/embor.2010.90
Subject(s) - g2 m dna damage checkpoint , dna damage , microbiology and biotechnology , plk1 , phosphorylation , cell cycle checkpoint , chek1 , cell cycle , biology , kinase , checkpoint kinase 2 , polo like kinase , regulator , cell cycle protein , nuclear protein , dna , protein kinase a , biochemistry , protein serine threonine kinases , cell , gene , transcription factor
In response to G2 DNA damage, the p53 pathway is activated to lead to cell‐cycle arrest, but how p53 is eliminated during the subsequent recovery process is poorly understood. It has been established that Polo‐like kinase 1 (Plk1) controls G2 DNA‐damage recovery. However, whether Plk1 activity contributes to p53 inactivation during this process is unknown. In this study, we show that G2 and S‐phase‐expressed 1 (GTSE1) protein, a negative regulator of p53, is required for G2 checkpoint recovery and that Plk1 phosphorylation of GTSE1 at Ser 435 promotes its nuclear localization, and thus shuttles p53 out of the nucleus to lead to its degradation during the recovery.