Premium
Direct interaction between hnRNP‐M and CDC5L/PLRG1 proteins affects alternative splice site choice
Author(s) -
Llères David,
Denegri Marco,
Biggiogera Marco,
Ajuh Paul,
Lamond Angus I
Publication year - 2010
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/embor.2010.64
Subject(s) - spliceosome , heterogeneous nuclear ribonucleoprotein , ribonucleoprotein , rna splicing , biology , heterogeneous ribonucleoprotein particle , microbiology and biotechnology , alternative splicing , genetics , messenger rna , rna , gene
Heterogeneous nuclear ribonucleoprotein‐M (hnRNP‐M) is an abundant nuclear protein that binds to pre‐mRNA and is a component of the spliceosome complex. A direct interaction was detected in vivo between hnRNP‐M and the human spliceosome proteins cell division cycle 5‐like (CDC5L) and pleiotropic regulator 1 (PLRG1) that was inhibited during the heat‐shock stress response. A central region in hnRNP‐M is required for interaction with CDC5L/PLRG1. hnRNP‐M affects both 5′ and 3′ alternative splice site choices, and an hnRNP‐M mutant lacking the CDC5L/PLRG1 interaction domain is unable to modulate alternative splicing of an adeno‐E1A mini‐gene substrate.