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TBCCD1, a new centrosomal protein, is required for centrosome and Golgi apparatus positioning
Author(s) -
Gonçalves João,
Nolasco Sofia,
Nascimento Rute,
Fanarraga Mónica Lopez,
Zabala Juan Carlos,
Soares Helena
Publication year - 2010
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/embor.2010.5
Subject(s) - centrosome , microbiology and biotechnology , cilium , golgi apparatus , basal body , centrosome cycle , biology , microtubule , midbody , microtubule organizing center , chemistry , cytokinesis , cell , cell division , cell cycle , flagellum , genetics , endoplasmic reticulum , gene
In animal cells the centrosome is positioned at the cell centre in close association with the nucleus. The mechanisms responsible for this are not completely understood. Here, we report the first characterization of human TBCC‐domain containing 1 (TBCCD1), a protein related to tubulin cofactor C. TBCCD1 localizes at the centrosome and at the spindle midzone, midbody and basal bodies of primary and motile cilia. Knockdown of TBCCD1 in RPE‐1 cells caused the dissociation of the centrosome from the nucleus and disorganization of the Golgi apparatus. TBCCD1‐depleted cells are larger, less efficient in primary cilia assembly and their migration is slower in wound‐healing assays. However, the major microtubule‐nucleating activity of the centrosome is not affected by TBCCD1 silencing. We propose that TBCCD1 is a key regulator of centrosome positioning and consequently of internal cell organization.