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microRNA/Argonaute 2 regulates nonsense‐mediated messenger RNA decay
Author(s) -
Choe Junho,
Cho Hana,
Lee Hyung Chul,
Kim Yoon Ki
Publication year - 2010
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/embor.2010.44
Subject(s) - argonaute , nonsense mediated decay , biology , microrna , gene silencing , messenger rna , p bodies , microbiology and biotechnology , untranslated region , rna induced silencing complex , regulation of gene expression , post transcriptional regulation , rna binding protein , gene expression , rna interference , translation (biology) , rna , gene , genetics , non coding rna , rna splicing
Imperfect base‐pairing between microRNA (miRNA) and the 3′‐untranslated region of target messenger RNA (mRNA) triggers translational repression of the target mRNA. Here, we provide evidence that human Argonaute 2 targets cap‐binding protein (CBP)80/20‐bound mRNAs and exon junction complex‐bound mRNAs and inhibits nonsense‐mediated mRNA decay (NMD), which is restricted tightly to CBP80/20‐bound mRNAs. Furthermore, microarray analyses reveal that a subset of cellular transcripts, which are expected to be targeted for NMD, is stabilized by miRNA‐mediated gene silencing. The regulation of NMD by miRNAs will shed light on a new post‐transcriptional regulation mechanism of gene expression in mammalian cells.