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Yox1 links MBF‐dependent transcription to completion of DNA synthesis
Author(s) -
GómezEscoda Blanca,
Ivanova Tsvetomira,
Calvo Isabel A,
AlvesRodrigues Isabel,
Hidalgo Elena,
Ayté José
Publication year - 2011
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/embor.2010.187
Subject(s) - dna replication , microbiology and biotechnology , origin recognition complex , g2 m dna damage checkpoint , biology , effector , repressor , transcription factor , eukaryotic dna replication , control of chromosome duplication , transcription (linguistics) , dna , checkpoint kinase 2 , cell cycle checkpoint , cell cycle , gene , genetics , linguistics , philosophy
When DNA replication is challenged cells activate a DNA synthesis checkpoint, blocking cell cycle progression until they are able to overcome the replication defects. In fission yeast, Cds1 is the effector kinase of this checkpoint, inhibiting M‐phase entry, stabilizing stalled replication forks and triggering transcriptional activation of S‐phase genes. The molecular basis of this last effect is largely unknown. The Mlu1 binding factor (MBF) complex controls the transcription of S‐phase genes. We purified novel interactors of the MBF complex and identified the repressor Yox1. When the DNA synthesis checkpoint is activated, Yox1 is phosphorylated, which abrogates its binding to MBF. MBF‐dependent transcription therefore remains active until cells are able to overcome this challenge.