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Uncovering new substrates for Aurora A kinase
Author(s) -
Sardon Teresa,
Pache Roland A,
Stein Amelie,
Molina Henrik,
Vernos Isabelle,
Aloy Patrick
Publication year - 2010
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/embor.2010.171
Subject(s) - computational biology , biology
Aurora A is a serine/threonine kinase that is essential for a wide variety of cell‐cycle‐related events, but only a small number of its substrates are known. We present and validate a strategy by which to identify Aurora A substrates and their phosphorylation sites. We developed a computational approach integrating various types of biological information to generate a list of 90 potential Aurora substrates, with a prediction accuracy of about 80%. We also demonstrated the specific phosphorylation of NUSAP (nucleolar and spindle‐associated protein) by Aurora A in vivo . Our results provide a means by which to develop an understanding of Aurora A function and suggest unexpected roles for this kinase.