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Role of the RNA/DNA kinase Grc3 in transcription termination by RNA polymerase I
Author(s) -
Braglia Priscilla,
Heindl Katrin,
Schleiffer Alexander,
Martinez Javier,
Proudfoot Nick J
Publication year - 2010
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/embor.2010.130
Subject(s) - termination factor , rna polymerase ii , exonuclease , transcription (linguistics) , rna polymerase i , polymerase , rna , microbiology and biotechnology , rna dependent rna polymerase , terminator (solar) , biology , rna polymerase ii holoenzyme , gene , genetics , gene expression , promoter , ionosphere , linguistics , philosophy , physics , astronomy
Transcription termination by RNA polymerase I in Saccharomyces cerevisiae is mediated by a ‘torpedo’ mechanism: co‐transcriptional RNA cleavage by Rnt1 at the ribosomal DNA 3′‐region generates a 5′‐end that is recognized by the 5′–3′ exonuclease Rat1; this degrades the downstream transcript and eventually causes termination. In this study, we identify Grc3 as a new factor involved in this process. We demonstrate that GRC3 , an essential gene of previously unknown function, encodes a polynucleotide kinase that is required for efficient termination by RNA polymerase I. We propose that it controls the phosphorylation status of the downstream Rnt1 cleavage product and thereby regulates its accessibility to the torpedo Rat1.