Premium
Polyadenylation and degradation of incomplete RNA polymerase I transcripts in mammalian cells
Author(s) -
Shcherbik Natalia,
Wang Minshi,
Lapik Yevgeniya R,
Srivastava Leena,
Pestov Dimitri G
Publication year - 2010
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/embor.2009.271
Subject(s) - polyadenylation , rna polymerase i , ribosomal rna , biology , microbiology and biotechnology , rna polymerase ii , polymerase , rna polymerase iii , rna , transcription (linguistics) , rna dependent rna polymerase , genetics , gene , gene expression , promoter , linguistics , philosophy
Most transcripts in growing cells are ribosomal RNA precursors (pre‐rRNA). Here, we show that in mammals, aberrant pre‐rRNA transcripts generated by RNA polymerase I (Pol I) are polyadenylated and accumulate markedly after treatment with low concentrations of actinomycin D (ActD), which blocks the synthesis of full‐length rRNA. The poly(A) polymerase‐associated domain‐containing protein 5 is required for polyadenylation, whereas the exosome is partly responsible for the degradation of the short aberrant transcripts. Thus, polyadenylation functions in the quality control of Pol I transcription in metazoan cells. The impact of excessive aberrant RNAs on the degradation machinery is an unrecognized mechanism that might contribute to biological properties of ActD.