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The structure of bacterial RNA polymerase in complex with the essential transcription elongation factor NusA
Author(s) -
Yang Xiao,
Molimau Seeseei,
Doherty Geoff P,
Johnston Elecia B,
MarlesWright Jon,
Rothnagel Rosalba,
Hankamer Ben,
Lewis Richard J,
Lewis Peter J
Publication year - 2009
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/embor.2009.155
Subject(s) - rna polymerase ii , transcription factor ii d , rna polymerase , rna polymerase ii holoenzyme , polymerase , elongation factor , transcription (linguistics) , termination factor , biology , transcription factor ii f , rna polymerase i , general transcription factor , rna , sigma factor , microbiology and biotechnology , rna dependent rna polymerase , genetics , dna , promoter , gene expression , gene , ribosome , linguistics , philosophy
There are three stages of transcribing DNA into RNA. These stages are initiation, elongation and termination, and they are well‐understood biochemically. However, despite the plethora of structural information made available on RNA polymerase in the last decade, little is available for RNA polymerase in complex with transcription elongation factors. To understand the mechanisms of transcriptional regulation, we describe the first structure, to our knowledge, for a bacterial RNA polymerase in complex with an essential transcription elongation factor. The resulting structure formed between the RNA polymerase and NusA from Bacillus subtilis provides important insights into the transition from an initiation complex to an elongation complex, and how NusA is able to modulate transcription elongation and termination.