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Triggering the formation of tasiRNAs in Arabidopsis thaliana : the role of microRNA miR173
Author(s) -
Felippes Felipe F,
Weigel Detlef
Publication year - 2009
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/embor.2008.247
Subject(s) - biology , arabidopsis thaliana , microrna , arabidopsis , gene , genetics , function (biology) , gene silencing , rna , mutant
Trans‐acting small interfering RNAs (tasiRNAs) comprise a class of endogenous small RNAs that are generated from TAS gene‐derived transcripts after these are cleaved at a microRNA (miRNA) target site. Arabidopsis thaliana has four families of TAS genes: miR173 triggers tasiRNA production from TAS1 and TAS2 , miR390 from TAS3 and miR828 from TAS4 . The two‐hit trigger model postulates that dual target sites in the same transcript are often sufficient to initiate tasiRNA production, but two hits are not always required for tasiRNA formation. Here, we characterize the function of miR173 in the formation of tasiRNAs from TAS1 transcripts, as well as the importance of the TAS1 and TAS3 transcript sequences outside the miRNA‐targeting sites for tasiRNA production. We show that tasiRNAs can be produced from heterologous transcripts containing miR173 or miR390 target sites, indicating that these trigger sequences are the only cis sequences essential for tasiRNA formation.