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Human mitotic chromosomes consist predominantly of irregularly folded nucleosome fibres without a 30‐nm chromatin structure
Author(s) -
Nishino Yoshinori,
Eltsov Mikhail,
Joti Yasumasa,
Ito Kazuki,
Takata Hideaki,
Takahashi Yukio,
Hihara Saera,
Frangakis Achilleas S,
Imamoto Naoko,
Ishikawa Tetsuya,
Maeshima Kazuhiro
Publication year - 2012
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/emboj.2012.35
Subject(s) - biology , chromatin , nucleosome , mitosis , microbiology and biotechnology , dna , genetics , biophysics
How a long strand of genomic DNA is compacted into a mitotic chromosome remains one of the basic questions in biology. The nucleosome fibre, in which DNA is wrapped around core histones, has long been assumed to be folded into a 30‐nm chromatin fibre and further hierarchical regular structures to form mitotic chromosomes, although the actual existence of these regular structures is controversial. Here, we show that human mitotic HeLa chromosomes are mainly composed of irregularly folded nucleosome fibres rather than 30‐nm chromatin fibres. Our comprehensive and quantitative study using cryo‐electron microscopy and synchrotron X‐ray scattering resolved the long‐standing contradictions regarding the existence of 30‐nm chromatin structures and detected no regular structure >11 nm. Our finding suggests that the mitotic chromosome consists of irregularly arranged nucleosome fibres, with a fractal nature, which permits a more dynamic and flexible genome organization than would be allowed by static regular structures.