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TNFα signals through specialized factories where responsive coding and miRNA genes are transcribed
Author(s) -
Papantonis Argyris,
Kohro Takahide,
Baboo Sabyasachi,
Larkin Joshua D,
Deng Binwei,
Short Patrick,
Tsutsumi Shuichi,
Taylor Stephen,
Kanki Yasuharu,
Kobayashi Mika,
Li Guoliang,
Poh HuayMei,
Ruan Xiaoan,
Aburatani Hiroyuki,
Ruan Yijun,
Kodama Tatsuhiko,
Wada Youichiro,
Cook Peter R
Publication year - 2012
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/emboj.2012.288
Subject(s) - biology , gene , chromatin , genetics , rna , microrna , computational biology , microbiology and biotechnology
Tumour necrosis factor alpha (TNFα) is a potent cytokine that signals through nuclear factor kappa B (NFκB) to activate a subset of human genes. It is usually assumed that this involves RNA polymerases transcribing responsive genes wherever they might be in the nucleus. Using primary human endothelial cells, variants of chromosome conformation capture (including 4C and chromatin interaction analysis with paired‐end tag sequencing), and fluorescence in situ hybridization to detect single nascent transcripts, we show that TNFα induces responsive genes to congregate in discrete ‘NFκB factories’. Some factories further specialize in transcribing responsive genes encoding micro‐RNAs that target downregulated mRNAs. We expect all signalling pathways to contain this extra leg, where responding genes are transcribed in analogous specialized factories.