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RIG‐I detects infection with live Listeria by sensing secreted bacterial nucleic acids
Author(s) -
Abdullah Zeinab,
Schlee Martin,
Roth Susanne,
Mraheil Mobarak Abu,
Barchet Winfried,
Böttcher Jan,
Hain Torsten,
Geiger Sergej,
Hayakawa Yoshihiro,
Fritz Jörg H,
Civril Filiz,
Hopfner KarlPeter,
Kurts Christian,
Ruland Jürgen,
Hartmann Gunther,
Chakraborty Trinad,
Knolle Percy A
Publication year - 2012
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/emboj.2012.274
Subject(s) - biology , nucleic acid , listeria , microbiology and biotechnology , listeria monocytogenes , bacteria , listeria infection , biochemistry , genetics
Immunity against infection with Listeria monocytogenes is not achieved from innate immune stimulation by contact with killed but requires viable Listeria gaining access to the cytosol of infected cells. It has remained ill‐defined how such immune sensing of live Listeria occurs. Here, we report that efficient cytosolic immune sensing requires access of nucleic acids derived from live Listeria to the cytoplasm of infected cells. We found that Listeria released nucleic acids and that such secreted bacterial RNA/DNA was recognized by the cytosolic sensors RIG‐I, MDA5 and STING thereby triggering interferon β production. Secreted Listeria nucleic acids also caused RIG‐I‐dependent IL‐1β‐production and inflammasome activation. The signalling molecule CARD9 contributed to IL‐1β production in response to secreted nucleic acids. In conclusion, cytosolic recognition of secreted bacterial nucleic acids by RIG‐I provides a mechanistic explanation for efficient induction of immunity by live bacteria.