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The Reb1‐homologue Ydr026c/Nsi1 is required for efficient RNA polymerase I termination in yeast
Author(s) -
Reiter Alarich,
Hamperl Stephan,
Seitz Hannah,
Merkl Philipp,
PerezFernandez Jorge,
Williams Lydia,
Gerber Jochen,
Németh Attila,
Léger Isabelle,
Gadal Olivier,
Milkereit Philipp,
Griesenbeck Joachim,
Tschochner Herbert
Publication year - 2012
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/emboj.2012.185
Subject(s) - biology , yeast , polymerase , rna polymerase ii , transcription factor ii d , genetics , rna polymerase , rna polymerase i , microbiology and biotechnology , rna , rna dependent rna polymerase , computational biology , gene , gene expression , promoter
Several DNA cis ‐elements and trans ‐acting factors were described to be involved in transcription termination and to release the elongating RNA polymerases from their templates. Different models for the molecular mechanism of transcription termination have been suggested for eukaryotic RNA polymerase I (Pol I) from results of in vitro and in vivo experiments. To analyse the molecular requirements for yeast RNA Pol I termination, an in vivo approach was used in which efficient termination resulted in growth inhibition. This led to the identification of a Myb‐like protein, Ydr026c, as bona fide termination factor, now designated Nsi1 (NTS1 silencing protein 1), since it was very recently described as silencing factor of ribosomal DNA. Possible Nsi1 functions in regard to the mechanism of transcription termination are discussed.