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ARF6 GTPase protects the post‐mitotic midbody from 14‐3‐3‐mediated disintegration
Author(s) -
Joseph Nimesh,
Hutterer Andrea,
Poser Ina,
Mishima Masanori
Publication year - 2012
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/emboj.2012.139
Subject(s) - biology , midbody , mitosis , microbiology and biotechnology , gtpase , small gtpase , cytokinesis , genetics , cell division , signal transduction , cell
In cytokinesis, there is a lengthy interval between cleavage furrow ingression and abscission, during which the midbody microtubule bundle provides both structural support for a narrow intercellular bridge and a platform that orchestrates the biochemical preparations for abscission. It is currently unclear how the midbody structure is stably maintained during this period. Here, we report a novel role for the ADP‐ribosylation factor 6 (ARF6) GTPase in the post‐mitotic stabilisation of midbody. Centralspindlin kinesin‐6/RhoGAP complex, a midbody component critical for both the formation and function of the midbody, assembles in a sharp band at the centre of the structure in a manner antagonised by 14‐3‐3 protein. We show that ARF6 competes with 14‐3‐3 for binding to centralspindlin such that midbodies formed by centralspindlin mutants that can bind 14‐3‐3 but not ARF6 frequently collapse before abscission. These data indicate a novel mechanism for the regulation of midbody dynamics in which ARF6 protects the compacted centralspindlin assembly from dissipation by 14‐3‐3.