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A MatP–divisome interaction coordinates chromosome segregation with cell division in E. coli
Author(s) -
Espéli Olivier,
Borne Romain,
Dupaigne Pauline,
Thiel Axel,
Gigant Emmanuelle,
Mercier Romain,
Boccard Frédéric
Publication year - 2012
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/emboj.2012.128
Subject(s) - biology , replisome , chromosome segregation , cell division , genetics , chromosome , ter protein , dna replication , origin of replication , dna , cell cycle protein , microbiology and biotechnology , cell , cell cycle , circular bacterial chromosome , gene
Initiation of chromosome segregation in bacteria is achieved by proteins acting near the origin of replication. Here, we report that the precise choreography of the terminus region of the Escherichia coli chromosome is also tightly controlled. The segregation of the terminus (Ter) macrodomain (MD) involves the structuring factor MatP. We characterized that migration of the Ter MD from the new pole to mid‐cell and its subsequent persistent localization at mid‐cell relies on several processes. First, the replication of the Ter DNA is concomitant with its recruitment from the new pole to mid‐cell in a sequential order correlated with the position on the genetic map. Second, using a strain carrying a linear chromosome with the Ter MD split in two parts, we show that replisomes are repositioned at mid‐cell when replication of the Ter occurs. Third, we demonstrate that anchoring the Ter MD at mid‐cell depends on the specific interaction of MatP with the division apparatus‐associated protein ZapB. Our results reveal how segregation of the Ter MD is integrated in the cell‐cycle control.