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Ubiquitylation of the initiator caspase DREDD is required for innate immune signalling
Author(s) -
Meinander Annika,
Runchel Christopher,
Tenev Tencho,
Chen Li,
Kim ChanHee,
Ribeiro Paulo S,
Broemer Meike,
Leulier Francois,
Zvelebil Marketa,
Silverman Neal,
Meier Pascal
Publication year - 2012
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/emboj.2012.121
Subject(s) - biology , innate immune system , ubiquitin , microbiology and biotechnology , signalling , caspase , immune system , caspase 8 , ubiquitin protein ligases , ubiquitin ligase , innate lymphoid cell , deubiquitinating enzyme , apoptosis , immunology , biochemistry , programmed cell death , gene
Caspases have been extensively studied as critical initiators and executioners of cell death pathways. However, caspases also take part in non‐apoptotic signalling events such as the regulation of innate immunity and activation of nuclear factor‐κB (NF‐κB). How caspases are activated under these conditions and process a selective set of substrates to allow NF‐κB signalling without killing the cell remains largely unknown. Here, we show that stimulation of the Drosophila pattern recognition protein PGRP‐LCx induces DIAP2‐dependent polyubiquitylation of the initiator caspase DREDD. Signal‐dependent ubiquitylation of DREDD is required for full processing of IMD, NF‐κB/Relish and expression of antimicrobial peptide genes in response to infection with Gram‐negative bacteria. Our results identify a mechanism that positively controls NF‐κB signalling via ubiquitin‐mediated activation of DREDD. The direct involvement of ubiquitylation in caspase activation represents a novel mechanism for non‐apoptotic caspase‐mediated signalling.