Premium
BAX inhibitor‐1 regulates autophagy by controlling the IRE1α branch of the unfolded protein response
Author(s) -
Castillo Karen,
RojasRivera Diego,
Lisbona Fernanda,
Caballero Benjamín,
Nassif Melissa,
Court Felipe A,
Schuck Sebastian,
Ibar Consuelo,
Walter Peter,
Sierralta Jimena,
Glavic Alvaro,
Hetz Claudio
Publication year - 2011
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/emboj.2011.318
Subject(s) - biology , autophagy , unfolded protein response , microbiology and biotechnology , endoplasmic reticulum , apoptosis , genetics
Both autophagy and apoptosis are tightly regulated processes playing a central role in tissue homeostasis. Bax inhibitor 1 (BI‐1) is a highly conserved protein with a dual role in apoptosis and endoplasmic reticulum (ER) stress signalling through the regulation of the ER stress sensor inositol requiring kinase 1 α (IRE1α). Here, we describe a novel function of BI‐1 in the modulation of autophagy. BI‐1‐deficient cells presented a faster and stronger induction of autophagy, increasing LC3 flux and autophagosome formation. These effects were associated with enhanced cell survival under nutrient deprivation. Repression of autophagy by BI‐1 was dependent on cJun‐N terminal kinase (JNK) and IRE1α expression, possibly due to a displacement of TNF‐receptor associated factor‐2 (TRAF2) from IRE1α. Targeting BI‐1 expression in flies altered autophagy fluxes and salivary gland degradation. BI‐1 deficiency increased flies survival under fasting conditions. Increased expression of autophagy indicators was observed in the liver and kidney of bi‐1 ‐deficient mice. In summary, we identify a novel function of BI‐1 in multicellular organisms, and suggest a critical role of BI‐1 as a stress integrator that modulates autophagy levels and other interconnected homeostatic processes.