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Structure of the Ire1 autophosphorylation complex and implications for the unfolded protein response
Author(s) -
Ali Maruf M U,
Bagratuni Tina,
Davenport Emma L,
Nowak Piotr R,
SilvaSantisteban M Cris,
Hardcastle Anthea,
McAndrews Craig,
Rowlands Martin G,
Morgan Gareth J,
Aherne Wynne,
Collins Ian,
Davies Faith E,
Pearl Laurence H
Publication year - 2011
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/emboj.2011.18
Subject(s) - biology , unfolded protein response , autophosphorylation , computational biology , genetics , microbiology and biotechnology , phosphorylation , endoplasmic reticulum , protein kinase a
Ire1 (Ern1) is an unusual transmembrane protein kinase essential for the endoplasmic reticulum (ER) unfolded protein response (UPR). Activation of Ire1 by association of its N‐terminal ER luminal domains promotes autophosphorylation by its cytoplasmic kinase domain, leading to activation of the C‐terminal ribonuclease domain, which splices Xbp1 mRNA generating an active Xbp1s transcriptional activator. We have determined the crystal structure of the cytoplasmic portion of dephosphorylated human Ire1α bound to ADP, revealing the ‘phosphoryl‐transfer’ competent dimeric face‐to‐face complex, which precedes and is distinct from the back‐to‐back RNase ‘active’ conformation described for yeast Ire1. We show that the Xbp1‐specific ribonuclease activity depends on autophosphorylation, and that ATP‐competitive inhibitors staurosporin and sunitinib, which inhibit autophosphorylation in vitro , also inhibit Xbp1 splicing in vivo . Furthermore, we demonstrate that activated Ire1α is a competent protein kinase, able to phosphorylate a heterologous peptide substrate. These studies identify human Ire1α as a target for development of ATP‐competitive inhibitors that will modulate the UPR in human cells, which has particular relevance for myeloma and other secretory malignancies.