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Nogo‐B receptor is necessary for cellular dolichol biosynthesis and protein N ‐glycosylation
Author(s) -
Harrison Kenneth D,
Park Eon Joo,
Gao Ningguo,
Kuo Andrew,
Rush Jeffrey S,
Waechter Charles J,
Lehrman Mark A,
Sessa William C
Publication year - 2011
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/emboj.2011.147
Subject(s) - dolichol , glycosylation , biology , biochemistry , biosynthesis , n linked glycosylation , receptor , glycoprotein , enzyme , glycan
Dolichol monophosphate (Dol‐P) functions as an obligate glycosyl carrier lipid in protein glycosylation reactions. Dol‐P is synthesized by the successive condensation of isopentenyl diphosphate (IPP), with farnesyl diphosphate catalysed by a cis ‐isoprenyltransferase ( cis ‐IPTase) activity. Despite the recognition of cis ‐IPTase activity 40 years ago and the molecular cloning of the human cDNA encoding the mammalian enzyme, the molecular machinery responsible for regulating this activity remains incompletely understood. Here, we identify Nogo‐B receptor (NgBR) as an essential component of the Dol‐P biosynthetic machinery. Loss of NgBR results in a robust deficit in cis ‐IPTase activity and Dol‐P production, leading to diminished levels of dolichol‐linked oligosaccharides and a broad reduction in protein N ‐glycosylation. NgBR interacts with the previously identified cis ‐IPTase hCIT, enhances hCIT protein stability, and promotes Dol‐P production. Identification of NgBR as a component of the cis ‐IPTase machinery yields insights into the regulation of dolichol biosynthesis.