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A multiprotein complex necessary for both transcription and DNA replication at the β‐globin locus
Author(s) -
Karmakar Subhradip,
Mahajan Milind C,
Schulz Vincent,
Boyapaty Gokul,
Weissman Sherman M
Publication year - 2010
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/emboj.2010.204
Subject(s) - biology , genetics , dna replication , locus (genetics) , transcription (linguistics) , origin recognition complex , dna , eukaryotic dna replication , computational biology , microbiology and biotechnology , gene , linguistics , philosophy
DNA replication, repair, transcription and chromatin structure are intricately associated nuclear processes, but the molecular links between these events are often obscure. In this study, we have surveyed the protein complexes that bind at β‐globin locus control region, and purified and characterized the function of one such multiprotein complex from human erythroleukemic K562 cells. We further validated the existence of this complex in human CD34+ cell‐derived normal erythroid cells. This complex contains ILF2/ILF3 transcription factors, p300 acetyltransferase and proteins associated with DNA replication, transcription and repair. RNAi knockdown of ILF2, a DNA‐binding component of this complex, abrogates the recruitment of the complex to its cognate DNA sequence and inhibits transcription, histone acetylation and usage of the origin of DNA replication at the β‐globin locus. These results imply a direct link between mammalian DNA replication, transcription and histone acetylation mediated by a single multiprotein complex.