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S6K1 is a multifaceted regulator of Mdm2 that connects nutrient status and DNA damage response
Author(s) -
Lai Keng Po,
Leong Wai Fook,
Chau Jenny Fung Ling,
Jia Deyong,
Zeng Li,
Liu Huijuan,
He Lin,
Hao Aijun,
Zhang Hongbing,
Meek David,
Velagapudi Chakradhar,
Habib Samy L,
Li Baojie
Publication year - 2010
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/emboj.2010.166
Subject(s) - p70 s6 kinase 1 , dna damage , biology , mdm2 , regulator , pi3k/akt/mtor pathway , microbiology and biotechnology , phosphorylation , cancer research , apoptosis , signal transduction , dna , genetics , gene
p53 mediates DNA damage‐induced cell‐cycle arrest, apoptosis, or senescence, and it is controlled by Mdm2, which mainly ubiquitinates p53 in the nucleus and promotes p53 nuclear export and degradation. By searching for the kinases responsible for Mdm2 S163 phosphorylation under genotoxic stress, we identified S6K1 as a multifaceted regulator of Mdm2. DNA damage activates mTOR‐S6K1 through p38α MAPK. The activated S6K1 forms a tighter complex with Mdm2, inhibits Mdm2‐mediated p53 ubiquitination, and promotes p53 induction, in addition to phosphorylating Mdm2 on S163. Deactivation of mTOR‐S6K1 signalling leads to Mdm2 nuclear translocation, which is facilitated by S163 phosphorylation, a reduction in p53 induction, and an alteration in p53‐dependent cell death. These findings thus establish mTOR‐S6K1 as a novel regulator of p53 in DNA damage response and likely in tumorigenesis. S6K1–Mdm2 interaction presents a route for cells to incorporate the metabolic/energy cues into DNA damage response and links the aging‐controlling Mdm2–p53 and mTOR‐S6K pathways.