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Sua5p a single‐stranded telomeric DNA‐binding protein facilitates telomere replication
Author(s) -
Meng FeiLong,
Hu Yan,
Shen Ning,
Tong XiaJing,
Wang Jianyong,
Ding Jianping,
Zhou JinQiu
Publication year - 2009
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/emboj.2009.92
Subject(s) - biology , telomere , telomere binding protein , dna replication , dna binding protein , dna , genetics , seqa protein domain , replication (statistics) , microbiology and biotechnology , control of chromosome duplication , virology , gene , transcription factor
In budding yeast Saccharomyces cerevisiae , telomere length maintenance involves a complicated network as more than 280 telomere maintenance genes have been identified in the nonessential gene deletion mutant set. As a supplement, we identified additional 29 telomere maintenance genes, which were previously taken as essential genes. In this study, we report a novel function of Sua5p in telomere replication. Epistasis analysis and telomere sequencing show that sua5 Δ cells display progressively shortened telomeres at early passages, and Sua5 functions downstream telomerase recruitment. Further, biochemical, structural and genetic studies show that Sua5p specifically binds single‐stranded telomeric (ssTG) DNA in vitro through a distinct DNA‐binding region on its surface, and the DNA‐binding ability is essential for its telomere function. Thus, Sua5p represents a novel ssTG DNA‐binding protein and positively regulates the telomere length in vivo .