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Accumulation of raft lipids in T‐cell plasma membrane domains engaged in TCR signalling
Author(s) -
Zech Tobias,
Ejsing Christer S,
Gaus Katharina,
de Wet Ben,
Shevchenko Andrej,
Simons Kai,
Harder Thomas
Publication year - 2009
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/emboj.2009.6
Subject(s) - t cell receptor , biology , lipid raft , sphingomyelin , lipid microdomain , microbiology and biotechnology , phosphatidylserine , phosphatidylethanolamine , raft , jurkat cells , phosphatidylcholine , membrane lipids , membrane , lipidome , t cell , biochemistry , lipidomics , signal transduction , chemistry , immunology , phospholipid , immune system , organic chemistry , copolymer , polymer
Activating stimuli for T lymphocytes are transmitted through plasma membrane domains that form at T‐cell antigen receptor (TCR) signalling foci. Here, we determined the molecular lipid composition of immunoisolated TCR activation domains. We observed that they accumulate cholesterol, sphingomyelin and saturated phosphatidylcholine species as compared with control plasma membrane fragments. This provides, for the first time, direct evidence that TCR activation domains comprise a distinct molecular lipid composition reminiscent of liquid‐ordered raft phases in model membranes. Interestingly, TCR activation domains were also enriched in plasmenyl phosphatidylethanolamine and phosphatidylserine. Modulating the T‐cell lipidome with polyunsaturated fatty acids impaired the plasma membrane condensation at TCR signalling foci and resulted in a perturbed molecular lipid composition. These results correlate the accumulation of specific molecular lipid species with the specific plasma membrane condensation at sites of TCR activation and with early TCR activation responses.