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The transcription factor OsNAC4 is a key positive regulator of plant hypersensitive cell death
Author(s) -
Kaneda Takashi,
Taga Yuri,
Takai Ryota,
Iwano Megumi,
Matsui Hiroyoshi,
Takayama Seiji,
Isogai Akira,
Che FangSik
Publication year - 2009
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/emboj.2009.39
Subject(s) - biology , programmed cell death , hypersensitive response , transcription factor , microbiology and biotechnology , dna fragmentation , fragmentation (computing) , gene , apoptosis , genetics , ecology
The hypersensitive response (HR) is a common feature of plant immune responses and a type of programmed cell death. However, little is known about the induction mechanism of HR cell death. We report that overexpression of OsNAC4 , which encodes a plant‐specific transcription factor, leads to HR cell death accompanied by the loss of plasma membrane integrity, nuclear DNA fragmentation and typical morphological changes. In OsNAC4 knock‐down lines, HR cell death is markedly decreased in response to avirulent bacterial strains. After induction by an avirulent pathogen recognition signal, OsNAC4 is translocated into the nucleus in a phosphorylation‐dependent manner. A microarray analysis showed that the expression of 139 genes including OsHSP90 and IREN , encoding a Ca 2+ ‐dependent nuclease, were different between the OsNAC4 knock‐down line and control line during HR cell death. During the induction of HR cell death, OsHSP90 is involved in the loss of plasma membrane integrity, whereas IREN causes nuclear DNA fragmentation. Overall, our results indicate that two important events occurring during HR cell death are regulated by independent pathways.