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Pretaporter, a Drosophila protein serving as a ligand for Draper in the phagocytosis of apoptotic cells
Author(s) -
Kuraishi Takayuki,
Nakagawa Yukiko,
Nagaosa Kaz,
Hashimoto Yumi,
Ishimoto Takashi,
Moki Takeshi,
Fujita Yu,
Nakayama Hiroshi,
Dohmae Naoshi,
Shiratsuchi Akiko,
Yamamoto Naoko,
Ueda Koichi,
Yamaguchi Masamitsu,
Awasaki Takeshi,
Nakanishi Yoshinobu
Publication year - 2009
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/emboj.2009.343
Subject(s) - library science , graduate students , medical science , fujita scale , engineering , medical education , medicine , computer science , geography , meteorology
Phagocytic removal of cells undergoing apoptosis is necessary for animal development and tissue homeostasis. Draper, a homologue of the Caenorhabditis elegans phagocytosis receptor CED‐1, is responsible for the phagocytosis of apoptotic cells in Drosophila , but its ligand presumably present on apoptotic cells remains unknown. An endoplasmic reticulum protein that binds to the extracellular region of Draper was isolated. Loss of this protein, which we name Pretaporter, led to a reduced level of apoptotic cell clearance in embryos, and the overexpression of pretaporter in the mutant flies rescued this defect. Results from genetic analyses suggested that Pretaporter functionally interacts with Draper and the corresponding signal mediators. Pretaporter was exposed at the cell surface after the induction of apoptosis, and cells artificially expressing Pretaporter at their surface became susceptible to Draper‐mediated phagocytosis. Finally, the incubation with Pretaporter augmented the tyrosine‐phosphorylation of Draper in phagocytic cells. These results collectively suggest that Pretaporter relocates from the endoplasmic reticulum to the cell surface during apoptosis to serve as a ligand for Draper in the phagocytosis of apoptotic cells.