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dMec: a novel Mi‐2 chromatin remodelling complex involved in transcriptional repression
Author(s) -
Kunert Natascha,
Wagner Eugenia,
Murawska Magdalena,
Klinker Henrike,
Kremmer Elisabeth,
Brehm Alexander
Publication year - 2009
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/emboj.2009.3
Subject(s) - biology , psychological repression , chromatin , chromatin remodeling , microbiology and biotechnology , genetics , transcription (linguistics) , dna , gene expression , gene , linguistics , philosophy
The ATP‐dependent chromatin remodeller Mi‐2 functions as a transcriptional repressor and contributes to the suppression of cell fates during development in several model organisms. Mi‐2 is the ATPase subunit of the conserved Nucleosome Remodeling and Deacetylation ( NuRD ) complex, and transcriptional repression by Mi‐2 is thought to be dependent on its associated histone deacetylase. Here, we have purified a novel dMi‐2 complex from Drosophila that is distinct from dNuRD. dMec ( dMEP‐1 complex ) is composed of dMi‐2 and dMEP‐1. dMec is a nucleosome‐stimulated ATPase that is expressed in embryos, larval tissues and adult flies. Surprisingly, dMec is far more abundant than dNuRD and constitutes the major dMi‐2‐containing complex. Both dNuRD and dMec associate with proneural genes of the achaete–scute complex. However, despite lacking a histone deacetylase subunit, only dMec contributes to the repression of proneural genes. These results reveal an unexpected complexity in the composition and function of Mi‐2 complexes.