Premium
Identification of a perinuclear positioning element in human subtelomeres that requires A‐type lamins and CTCF
Author(s) -
Ottaviani Alexandre,
SchluthBolard Caroline,
RivalGervier Sylvie,
Boussouar Amina,
Rondier Delphine,
Foerster Andrea M,
Morere Julia,
Bauwens Serge,
Gazzo Sophie,
CalletBauchu Evelyne,
Gilson Eric,
Magdinier Frédérique
Publication year - 2009
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1038/emboj.2009.201
Subject(s) - ctcf , lamin , biology , subtelomere , genetics , telomere , nuclear lamina , insulator (electricity) , genome , genomic organization , gene , microbiology and biotechnology , conserved sequence , chromatin , peptide sequence , nuclear protein , gene expression , transcription factor , enhancer , electrical engineering , engineering
The localization of genes within the nuclear space is of paramount importance for proper genome functions. However, very little is known on the cis ‐acting elements determining subnuclear positioning of chromosome segments. We show here that the D4Z4 human subtelomeric repeat localizes a telomere at the nuclear periphery. This perinuclear activity lies within an 80 bp sequence included within a region known to interact with CTCF and A‐type Lamins. We further show that a reduced level of either CTCF or A‐type Lamins suppresses the perinuclear activities of D4Z4 and that an array of multimerized D4Z4 sequence, which has lost its ability to bind CTCF and A‐type Lamins, is not localized at the periphery. Overall, these findings reveal the existence of an 80 bp D4Z4 sequence that is sufficient to position an adjacent telomere to the nuclear periphery in a CTCF and A‐type lamins‐dependent manner. Strikingly, this sequence includes a 30 bp GA‐rich motif, which binds CTCF and is present at several locations in the human genome.